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Melatonin activates NRF2, which is considered a defense mechanism against ROS as it controls the expression of a collection of genes involved in antioxidant defenses and inflammatory responses 85,86,87,88

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The resulting mixture was combined with 80 l of 25 M N-acetylcysteine (internal standard), reduced using 20 l of 1.58 M N-ethylmorpholine and 8 l of 25 mM tris-(2-carboxyethy l) phosphine, and labeled with 8 l of 30 mM monobromobimane