Immunohistochemical staining of A showed that no visible A plaque was found after 4-week ethanol exposure in both cortex and hippocampus of WT and APP/PS1 mice (Fig
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Fluorescence images were acquired using an inverted fluorescence microscope
On the day of assay 2 g per well of the cell membrane was incubated with furimazine (1:1000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)
In addition to the tertiary structures that will be displayed, attention must also be given to the way in which peptide structures may aggregate to form dimeric, trimeric and tetrameric clusters
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