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Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min, and the clarified cell lysate was directly used for biocatalytic reactions

[DOI] [PubMed] [Google Scholar] 82.Dosenko V.E., Zagoriy V.Y., Haytovich N.V., Gordok O.A., Moibenko A.A

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Antioxidants (Basel, Switzerland), 10(3), 364

Ascorbate is oxidized to dehydroascorbate in plasma, which is recycled back to ascorbate by GSH as well as by several enzyme systems in erythrocytes, neutrophils, endothelial cells and hepatocytes