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Between 1% and 10% of the lysate was retained as input control, and the remaining lysate was used for immunoprecipitation

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DOI: 10.1016/j.archoralbio.2013.03.017 21

Finally, microbial functional profiling was carried out using HUMAnN 3.5 [34], through which the gene families were grouped into one feature table, normalized into copies per million (CPM), and further grouped in Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs [35,36,37], and pathways

To further verify the autophagy pathway, macrophages were labeled with an RFP-GFP-LC3 probe (LC3 thereafter)