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3 Results 3.1 Nicotine alleviated HFD-induced obesity and related metabolic disorders To investigate the effect of nicotine on HFD-induced obesity, HFD-fed C57BL/6J mice were randomly divided into two groups that were orally administrated nicotine (Nic, 0.5 mg/kg) or saline (negative control) daily for 12 weeks, respectively (Figure 1A)

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