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Subcutaneous administration in normal and metabolically dysregulated mice significantly inhibits NNMT activity in metabolically active tissues (adipose, liver, muscle), increases systemic NAD+ levels, enhances mitochondrial function, and improves energy metabolism

Their white epididymal and brown interscapular adipose tissues were dissected, weighed, and frozen in liquid nitrogen for later RNA analysis

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Larger needles cause more tissue displacement and tend to produce more discomfort

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