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The first strand cDNA was synthesized by SuperscriptII (ThermoFisher Scientific) and random primers at 42 C for 15 mins, followed by second strand synthesis at 16 C for 1 h

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One hour after l-carnitine, the norepinephrine requirements started to decrease, the patient began to improve and was subsequently extubated successfully without apparent sequelae in less than 4 days

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