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Transferring to nasal spray bottle Choosing nasal spray bottle: Capacity: 10-15ml ideal Pump type: Metered dose pump (0.1ml per spray) Material: Glass or quality plastic Where to buy: Amazon, pharmacy supply, medical supply sites Cost: $5-15 for bottle Transfer process: Clean empty nasal spray bottle thoroughly Remove pump/sprayer from bottle Using syringe, draw reconstituted PT-141 Slowly inject into spray bottle (or use small funnel) If multiple vials needed, repeat Replace pump/sprayer Prime pump (2-3 test sprays into air) Label bottle with concentration and date Refrigerate when not in use How much to make: 10mg PT-141 = 1ml at 10mg/ml One dose = 3-5mg = 0.3-0.5ml 10mg vial = 2-3 nasal doses Make multiple vials if regular use Shelf life: 28-30 days refrigerated Calculating total solution needed: Storage after preparation: Keep refrigerated 2-8C always Never freeze (destroys peptide) Protect from light (store in box/drawer) Use within 28-30 days Discard if cloudy or particles appear Use our peptide calculator for dose calculations

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Stress Reporter Assay of AQ-QI and DEAQ-QI To identify the stress response pathways upon AQ-QI and DEAQ-QI exposure, oxidative stress response (SRXN1-GFP), ER stress response (CHOP-GFP), inflammation response (ICAM1-GFP), and DNA damage response (p21-GFP) pathways were measured with a high-content image-based BAC-GFP stress responses reporter assay (Wink et al., 2014, 2017)

Sterile as well as non-sterile medications can be mass-produced in a facility that is designated as 503B

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