This study explored the application of DIA (MSe) and DDA (fast-DDA) in liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics using Panax genus samples
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Nilsen, J., Sandlie, I., Roopenian, D
Metabolites that significantly correlated with PROMIS-Fatigue T -scores were overrepresented by the steroid hormone biosynthesis pathway (KEGG ID: M00107), including androgen and estrogen metabolism (SMPDB ID: SMP0000068) and androstenedione metabolism (SMPDB ID: SMP0030406) (Figure 4C), which included androsterone sulfate ( r = 0.26, p = 0.0009), epiandrosterone sulfate ( r = 0.25, p = 0.0016), etiocholanolone glucuronide ( r = 0.19, p = 0.018), and dehydroepiandrosterone sulfate (DHEA-S) ( r = 0.18, p = 0.023)
Reasons include: Poor gut absorption Chronic inflammation Long-standing deficiencies Stress-related nutrient depletion Lifestyle-induced hormonal imbalances This is where functional medicine becomes crucial
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