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A 2015 review published in BioMed Research International by Pickart, Vasquez-Soltero, and Margolina provided a comprehensive overview of GHK-Cu as a natural modulator of multiple cellular pathways in skin regeneration

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10.1038/ng.2528 32 CorinaA.Rangel-ZigaO

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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock