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To prepare the FCR reagent, 0.1 g of sodium tungstate and 25 g of sodium molybdate are dissolved in 700 mL of distilled water, followed by acidification with 50 mL of concentrated HCl and 50 mL of 85% orthophosphoric acid (HPO)

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After washing twice with PBS, 2 M DCFH-DA was added and cells were incubated for 30 min at 37 C