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Agonist-induced activation of G protein-coupled receptors (pharmacomechanical coupling): Agonists such as drugs, hormones and/or neurotransmittersfor example, carbachol (in airway and gastrointestinal smooth muscle), phenylephrine (in vascular smooth muscle), and oxytocin (in uterine smooth muscle)can trigger the phospholipase C 1 (PLC 1) pathway by activation of their receptors, caus-ing calcium release from the sarcoplasmic reticulum mediated by inositol 1,4,5-trisphosphate (IP 3 ) and, subsequently, an influx of these ions through Cav in the cell membrane, in processes called phasic and tonic contraction, respectivel (Figure 4)

2, addition of 1.63.2 mM glutathione showed saturated activity enhancement