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Flow cytometry of tumor infiltrating immune cells Mice were euthanized, and tumors were mechanically dissociated and digested in a mixture of enzymes (0.1% collagenase type IV, 0.01% hyaluronidase, 1% FBS, 20 U/mL DNase I dissolved in HBSS buffer) for 30 minutes at 37 C

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GIP receptor stimulation supports metabolic efficiency