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Nutrients 11 , 931 (2019)

lysine has a predominant positive net charge below pH 10.0, thus for cation exchange the pH elution gradient with a solvent comprising an organic modifier could begin below pH 10.0 to elute a target GLP-1 moiety acylated in the side chain of the lysine residue and end the gradient above pH 10.0 thereby subsequently removing an undesired non-acylated form

This persistence improvement is strongly correlated with resolving drug shortages, helping experts predict what GLP-1 obesity treatment may look like now that drug supply is improving