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Undeterred, Knudsen plunged straight back into tweaking the peptide, which she says the company's management gave 'only one more shot' (Molteni)

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A transcriptomic based deconvolution framework for assessing differentiation stages and drug responses of AML

Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

It provided the scale and methodological rigor that earlier studies lacked

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