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First, we measure the fluorescence response of TPE-DCV to different amino acids in the detection medium (Figure 3)

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The aqueous mobile phase (A) consisted of H2O:ACN: FA:PFHA (99.3:0.5:0.1:0.1 v/v/v/v), and the organic mobile phase (B) consisted of H2O:FA (99.9:0.1 v/v)

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