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General cell biology methods RPMI-1640 medium, DMEM, Iscoves modified Dulbeccos medium (IMDM), FBS, penicillinstreptomycin (10,000 U per ml sodium penicillin G and 10,000 g ml 1 streptomycin), trypsinEDTA solution (1) and PBS (1) were purchased from Gibco (Invitrogen)

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3e), and coordination was only marginally improved on a medium (Fig