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Front Endocrinol (Lausanne) (2022) 13:800714

The PCR conditions were as follows: a 5 min initial denaturation at 95 C, 28 cycles of denaturation at 95 C (30 s), annealing at 55 C (30 s), and elongation at 72 C (45 s), followed by a final extension at 72 C for seven min

Another study evaluating insulin concentrations only during the first ten days postpartum similarly found that the rhythm was characterized by a decline during the night and an increase in the morning [60]

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doi: 10.1001/jamanetworkopen.2023.4625 13

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