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The short circuit current (I sc ) is also often calculated during electrophysiological measurements and refers to the amount of current needed to force the PD to 0 mV

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After 30 min incubation at room temperature, the treated lysates were further divided into smaller aliquots (50 l), which were then heated at different temperatures for 3 min on a Veriti thermal cycler (Applied Biosystems) followed by cooling for 3 min to room temperature

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