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After denaturation in Laemmli buffer (Tris 40 mM, DTT 100 mM, sodium dodecyl sulfate 2% (v/v), glycerol 5% (v/v), bromophenol blue 0.075% (m/v), pH 6.8), proteins were separated on a 4-12% Criterion XT Precast Gel (Bio-Rad) in XT MOPS buffer (Bio-Rad) at 120 V for 2 h
Reconstitution solution often refers to bacteriostatic water in peptide research contexts
The choice of route aims to balance effect size, convenience, and side-effect risk
We can't stress this enough: the integrity of your research hinges on the accuracy of your preparation
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