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doi: 10.1007/s11920-019-1021-4

Shown are the normalized values from two replicates with swapped SILAC labeling and the log2 of the average thereof

1 HNMR (300 MHz, DMSO- d 6 ,ppm): H 12.46 (s, 1H, hydrazine-N H ), 11.85 (s, 1H, 4-bromobenzylidene-N=C H ), 8.43 (s, 1H, 4-hydroxy-3-methoxy benzohydrazide-O H ), 7.90 (s, 1H, 4-hydroxy-3-methoxy benzohydrazide-C 2 - H ), 7.68 (m, 4H, 4-bromobenzylidene C 2 , 3,5,6 - H ), 6.53 (brs, 2H, 4-hydroxy-3-methoxy benzohydrazide-C 5,6 - H ), 3.80 (s, 3H, 4-hydroxy-3-methoxy benzohydrazide-OC H 3 )

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Signs of Low Glutathione Levels Low glutathione levels can contribute to a range of health issues, including: Chronic fatigue Increased susceptibility to infections Worsened symptoms of chronic diseases Accelerated aging Neurological conditions such as Parkinsons disease Macular degeneration These issues highlight the importance of maintaining optimal glutathione levels, either through lifestyle changes or supplementation

2.8 DNA fragmentation assay The DPA assay is a colorimetric and quantitative technique used to evaluate DNA fragmentation in T-47D and resistant T-47D cells treated with BV, DOX, and BV + DOX