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To measure saturation binding for FITC- or TMR-labelled exendin-4 and exendn-4-C16 directly, these ligands were added to cells and incubated as above, followed by measurement of TR-FRET signal using the following settings: FITC ligands: ex 340 nm, em 520 and 620 nm, auto-cutoff, delay 50 s, integration time 300 s

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