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CO-IP experiments were performed using Dynabeads M-270 Epoxy CO-IP kit according to the manufacturers recommendation, with modification in cell extraction buffer composition (1X IP buffer provided in kit, 100 mM NaCl, PMSF, 0.1% Octyl-d-glucoside)

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Cell pellets were resuspended in ice-cold buffer (2530 mL/L) containing 150 mM NaCl, 20 mM Tris (pH 8.0) and 1X Protease Inhibitors Cocktail (Roche)

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Wangchuk et al., 2019