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Long-term maintenance dosing remains stable for many patients once therapeutic goals are achieved, though periodic reassessment ensures continued appropriateness of therapy

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To test this prediction, we mutated VadK-His422 to alanine, checked that VadK His422Ala was folded in a similar manner to VadK WT by circular dichroism (CD) (Appendix Fig

The sample is then sent to a laboratory to measure the folate concentration in the serum or red blood cells