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Safranin O Staining Pellets were fixed overnight at 4C in 10% buffered formalin (Fisher Chemical, Hampton, NH, United States), dehydrated in gradient ethanol, cleared in xylene, and then embedded in paraffin

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No evidence of acute toxicity has been observed at standard research doses, though long-term effects on cellular proliferation remain under investigation

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Third, consider sequential introduction rather than simultaneous initiation, which allows attribution of effects and side effects to specific components