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At the end of the experiment hECs were lysed in DW supplemented with Triton X-100 detergent (10 mg/ml)

Paolella, L

The two ingredients have different biological roles, supplement positioning, and formula considerations

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Avoid freezing

Modules correlated with the KO genotype were enriched for neuronal and synaptic genes involved in vesicle-mediated transport, neurotransmitter secretion, and GTPase activity, suggesting that RAMP deletion disrupts synaptic signaling networks critical for metabolic regulation [2]