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If you have a history of alcohol use disorder: Alcohol should generally be avoided

Blots were further incubated with anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (1:5000, 31460, Thermo Scientific) for 2 h at room temperature, and immunoreactive bands were detected using the enhanced chemiluminescence (ECL) method in a ChemiDoc XRS System (BioRad) with Image Lab software