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(17)Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val--2010(TFA/EDT/H 2 O/Thio90-95/2-5/2-5/1-5)10-501-5-2010161024BPC157 (17) Take the dried Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin Pour into a glass cutting bottle, add the peptide cutting reagent (TFA/EDT/H 2 O/Thio=90-95/2-5/2-5/1-5, volume ratio ), reacted at 10C-50C for 1-5 hours, filtered to remove the resin, added -20-10C glacial ether to precipitate, centrifuged to collect the precipitate, washed 1-6 times with ether, and freeze-dried at low temperature for 10-24 hours to obtain Crude product of BPC157 peptide

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Monier BC, Aronsson DD, Sun M

doi: 10.1038/s42255-023-00931-7 55 ZhangQDelessaCTAugustinRBakhtiMColldenGDruckerDJet al