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Methods Materials Chemicals were ordered from Sigma, ABCR, Carl Roth, Lucerna Chem, Plantmetachem or Formedium, and were used without further purification

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Next, cells were collected and stained with the Alexa Fluor 647conjugated Phospho-Histone H2A.X (Ser139), or Cleaved PARP (Asp214) (D64E10) XP rabbit mAb and Anti-Rabbit IgG (H + L) F(ab) fragment antibodies (Cell Signaling, #5625 and #4412, respectively) after fixation and permeabilization with 100% methanol

Here are the results from each clinical trial

Inflammation in atherosclerosis: pathophysiology and mechanisms