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After 24 hours of treatment, cells were incubated for 4 hours at 37C with Aquabluer (Boca Scientific, #6015) and fluorescence intensity was measured at 540ex/590em via a SpectraMax M series microplate reader and normalized to the average of control wells

2022 Jul;352:88-95

It works through an entirely different mechanism, targeting amylin receptors rather than GLP-1 receptors alone, and that means its side effect profile carries its own unique characteristics that deserve careful examination

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