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The inhibition was dose-dependent and described as noncompetitive, meaning copper didnt block the substrate directly, but rather altered the enzymes activity

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The 2 mg 4 mg 8 mg 12 mg protocol was the most effective and forms the basis for Phase 3 trials (TRIUMPH programme)

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The synthesis of cDNA was done using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA) with 1 g RNA, and the qPCR was conducted using AriaMx Real-Time PCR systems (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturers instructions