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Comparing the sgRNA distribution in the non-treated cells with the library plasmid stock showed that several sgRNAs targeting essential control genes and essential membrane transporters led to a growth reduction (Fig S2B), confirming a high KO efficiency in this cell line and experimental setup

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81770327, 81100173) and Capital Health Research and Development of Special Fund (M2022038, China)

10.1038/s41419-019-1564-7 Cell Death Dis