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Traditional methods for studying molecular interactions include affinity purification, the yeast two-hybrid system [2, 3], the bimolecular fluorescence complementation assay (BiFC) [4,5,6], the glutathione S-transferase pull-down assay (GST pull-down assay) [7, 8], and coimmunoprecipitation (Co-IP) [9]

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This results in most patients being diagnosed at advanced stages, contributing to the poor prognosis and low five-year survival rate, making it known as the king of cancer. For patients with resectable PC, early surgical intervention followed by adjuvant therapy remains the preferred approach, offering potential cure [267]