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The beginning and advancement of ferroptosis are primarily governed by lipid ROS, ferrous iron, and GPX4 activity, where increased ferrous iron levels and reduced GPX4 activity signify the commencement of ferroptosis [79]

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This hypothesis is supported by Seahorse analysis, which revealed that CI inhibition by Rot treatment significantly reduced basal respiration, ATP-linked respiration, maximal respiration, and spare respiratory capacity, but not proton leak (Fig