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We measured the emission of hGrx1-roGFP2 (505515 nm) after excitation at 390 and 480 nm in a plate reader (M200, Tecan) with optimal read setting

Bottom line: Effectiveness is determined by cellular uptake, not the number on the front of the bottle

Subcutaneous injection technique involves cleaning the injection site with alcohol, pinching skin to create a fold, inserting the needle at a 45-90 degree angle, and injecting slowly

In the absence of these bases, the outcome might be minimal

The RDL receptor is a member of Cys-loop ligand-gated ion channel superfamily with a N-terminal extracellular domain for GABA binding