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As shown in Figure 7A, MAPKAPK2 protein levels were markedly reduced by si-MAPKAPK2 transfection, whereas re-expression of MAPKAPK2 effectively restored its protein abundance, confirming the efficiency of both knockdown and rescue

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Results Increased severity of liver injury and mortality of mice with hepsin deficiency during the early period after acetaminophen exposure To investigate the potential physiological role of endogenous hepsin in the early response to APAP-induced liver injury, we analyzed hepsin expression in wild-type mouse liver lysates following APAP treatment using western blotting (Fig