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Funding This work was supported by the Italian Ministry of Health (Ricerca Corrente 2021 to A.A.)

Rotate between these sites with each injection to prevent lipodystrophy, which is the development of hard lumps or fat loss at repeatedly used injection sites

Using 96372 for a chemotherapy agent triggers a medical-necessity denial

To reduce bruising, apply gentle pressure (do not rub) for 10-15 seconds after injection, ice the site if needed, and be aware that blood thinners and supplements like fish oil increase bruising tendency

as this was exploratory there were no study size decisions made a priori

Regulatory and Ethical Considerations Responsible epithalon research requires attention to regulatory guidelines and ethical standards