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Metformin has also been shown to have an anti-depressant effect in a chronic-restraint stress model of depression in mice, which is blocked by genetic knockdown of hippocampal AMPK catalytic subunits ( Prkaa1 and Prkaa2 ) [136]

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In the denaturing step, each 4 l sample was mixed with 18 l of an 8 M guanidine hydrochloride (cas 50-01-1, Carl Roth, #0037.1) solution

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