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Description

During anaplerosis, mitochondrial glutamate dehydrogenase 1 (GLUD1) plays a key role by catalyzing the conversion of glutamate to alpha-ketoglutarate (-KG) and releasing ammonia, which regulates autophagy and neutralizes the intracellular pH in cancer cells 19,20

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The 4 um thick paraffin sections were then dehydrated, stained with methylene blue, and washed with distilled water, followed by a repeat of dehydrating and washing the sections in order to prepare for a Nissl staining trial

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