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In vivo imaging revealed no significant difference in barin fluorescence intensity between the fasting mice and normal mice, further confirming that ITP-mediated brain targeting is independent of insulin-induced IR endocytosis in vivo (Supplementary Fig

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The current study not only includes multilayered in vitro and in vivo validation but also incorporates clinical studies, ultimately confirming that NAC supplementation can significantly enhance OI efficacy with sequential LE and uFSH in women with PCOS