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The sequence of events in microvesicle and nanovesicle formation and release illustrates that cellular aging as evident from increasing amounts of oxidized hemoglobin, aggregated band 3 protein and surface-bound IgG appears to induce an entrapment of these irreversible endproducts in lipid raft-containing microvesicles that are rapidly cleared

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Briefly, fecal samples were suspended in sterile distilled water by vigorous shaking, and the supernatant was transferred to a 0.5 ml tube containing glass beads (0.1 mm diameter), before being spun at 5,000 rpm for 3 min in a mini-bead beater (BioSpec Products, Bartlesville, OK, USA)