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Plasma semaglutide concentrations were measured in plasma by a validated liquid chromatography-mass spectrometry assay following protein precipitation (Celerion Switzerland AG, Fehraltorf, Switzerland) using previously reported methods [18, 20] with a lower limit of quantification of 0.729 nmol/L

D.EberhartT.Flckiger-MangualS.VukolicA.et al (2019)

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Rats were kept in a temperature-controlled room at 22 2 o C on a 12-h light/dark cycle with free access to food and water