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Immediately prior to experimentation, the stock solution was diluted with sterile saline to a concentration of 50 mg/kg (final DMSO concentration 1%) 31,32

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It was previously thought that prolactin was only produced by lactotrophs in the anterior pituitary, but recent literature indicates prolactin is also produced by the central nervous system (CNS), adipose tissue, immune system, uterus, mammary glands, and prostate gland ( During pregnancy and lactation, prolactin plays an important role in systemic signaling