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As a result, diverse glial immune responses and microbiota configurations may have emerged, leaving lasting biological imprints that could represent the evolutionary origins of aging and disease

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

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K., Zampeli, V

The vast majority 90 to 95 percent have type 2 diabetes, a condition primarily due to lifestyle factors, like poor diet and limited physical activity

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