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Under cooling in ice bath, 26.88 g of Fmoc-Gly-OH and 12.03 g of HOBt were dissolved in a mixed solvent of DMF and DCM

( D ) Immunofluorescence images showing colocalization of PAR2 and TMPRSS2 in the mouse small intestine

After one-week acclimatization, the mice were then randomly separated into four groups ( n = 8 per group) under indicated treatments for 15 weeks: (i) mice fed with normal control diet (NCD, D12450B, Research Diet)