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Peer review Peer review information Nature Reviews Endocrinology thanks Thomas Lutz and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Injected oocytes were stored at 18 C in ND96 storage buffer (96 mM NaCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM hemisodium-HEPES, pH 7.4 supplemented with 50 g/ml gentamycin, 2.5 mM sodium pyruvate, 50 g/mL tetracycline and 0.5 mM theophylline) for three days to allow the expression of hOCTN2 on the oocyte plasma membrane

Studies and clinical experience show that lipotropic injections work best when coupled with a calorie-restricted diet, regular resistance or aerobic exercise, and behavior-change coaching rather than as stand-alone therapy