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Western blot assay Gastrocnemius muscles were collected for the detection of NLRP3, GSDMD-FL, GSDMD-N, IL-1, IL-18, CD31, and VEGF-, while HUVECs and THP-1 cells were collected for the detection of GSDMD-FL, GSDMD-N, IL-1, IL-18, and CCL11

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