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206 In addition, expression of the mitochondrial isoform of phosphoenolpyruvate carboxykinase, involved in gluconeogenesis from non-carbohydrate carbon substrates such as pyruvate, lactate, glycerol and glucogenic amino acids, was significantly increased, 499 indicating an elevated demand for free glucose through enhancing astrocytic glucose metabolism in GDI1 -null mice

A Gatan K3 direct electron camera operating in correlated double sampling (CDS) mode equipped post a Gatan Quantum energy filter (Gatan, Pleasanton, CA, USA) was to record movies with a total dose of ~60 e per 2

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