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The reaction product, N-Hcy-Lys, was purified by HPLC using a reverse phase X Bridge Prep C18 preparative column (19 100 mm, 5 m) from Waters (Milford, MA, USA)
(Clin Transl Med, 2021) in 24 older adults (aged 61-80) found that supplementation with GlyNAC (glycine 100 mg/kg/day + NAC 100 mg/kg/day, approximately 7 g each for a 70 kg person) for 16 weeks corrected glutathione deficiency and improved multiple hallmarks of aging including oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, body composition, exercise capacity, and cognition [10]
The authors of the study collected urine and blood specimens from the participants and performed MRS scans on their brains prior to the first dose of sulforaphane and within four hours of the final dose
