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doi: 10.1016/0006-291x(87)91011-4

Full recovery depends on injury type and severity

RNA quality was assessed by Agilent 2100 Bioanalyzer using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and RNA quantification was performed using ND-2000 Spectrophotometer (Thermo Inc., DE, USA)

RNA isolation and qRT-PCR Total RNA from the tissues and cells was extracted using the TRIzol method (Takara, Japan)